Item Weight: pounds; Shipping Weight: 12 ounces; ASIN: B00IA6VY0Q; UPC: ; Average Customer Review: Be the first to review this item. Trim7 (Myc-DDK-tagged) - Mouse tripartite motif-containing 7 (Trim7) but a complete review of all prevailing variants is recommended prior to use. transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. , Knockdown of tripartite motif containing 7 (TRIM7) by siRNA inhibits . E3 ubiquitin-protein ligase TRIM7 Ex{3}LL splice variant In isoform 2. splice.


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NM_(TRIM7):cG>A AND Malignant melanoma - ClinVar - NCBI

While we cannot exclude entirely the possibility of off target modifications in addition to on target cleavage, trim7 2 spec believe it represents a relatively low and controllable risk, as a number of recent publications have demonstrated that the CRISPR-Cas9 system is to be highly specific e.

Further trim7 2 spec this a collaborator group have published a paper where they examine off-targets in the HAP1 cell line and observe very low frequencies. At Horizon, to mitigate the risk still further our in-house selection algorithms warrant that only those guide RNAs with minimal predicted off-target trim7 2 spec in the human genome are chosen.

Further to this, scientists can control their experiments through the use of multiple, independent clones or rescue of the knockout with a wild-type cDNA.

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I have never worked with HAP1 cells before. How do I know if they look correct? Our Technical Support team is happy to provide a trim7 2 spec inspection of your culture if you send an image to technical horizondiscovery.

MRL4 – Trim7 (NM_) Mouse Tagged ORF Clone | OriGene

Why are there multiple HAP1 cell lines listed for my gene of interest? The differences between them is often the number of bases that are inserted or deleted.

If you click on trim7 2 spec product pages for each of the clones and trim7 2 spec down the page, there is a pdf link with the datasheets attached.

This describes the location of the frameshift and you can then determine which is more suitable for your needs.

Are HAP1 cell lines genetically stable i. We have observed that haploid cell lines will spontaneously diploidise over time.


If a gene has been knocked out, diploidisation will not affect knockout status, but to minimise the potential impact of diploidisation one of the following approaches trim7 2 spec recommended: Work with cells at very low passage. Prepare stocks of low passage cells and revive frequently.

Trim7 2 spec pdf to jpg - PDF Files

Culture knockout and wild type cells in parallel and allow genomes to stabilise. Then use these high passage cells trim7 2 spec experimental analyses. How do I know if HAP1 cells are suitable for my studying a particular biological pathway?

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